Cytochrome P450 monooxygenase (CYP)

Fa'amatalaga Puupuu:

E uiga ile CYP

ES-CYPs: O se vasega o redox enzymes ma mercaptan-heme e avea ma nofoaga autu e faʻamalosia ai le faʻafeiloaʻi o le okesene.O okesene fa'agata ma e mana'omia le heme e fa'agaoioi ai le okesene.

Cytochrome P450 monooxygenase faʻamalosia le faʻamaʻiina, epoxidation ma le hydroxylation o alkyl, hydroxylation ma le faʻamaʻiina o le ammonia, faʻamaʻiina o le sulfur, dealkylation (oxygen, sulfur, ammonia), oxidative dehydrogenation, deamination ma dehalogenation.
E 8 ituaiga o cytochrome P450 monooxygenase oloa (Numera pei ES-CYP-101～ES-CYP-108) atiaʻe e SyncoZymes.

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Fa'amatalaga Oloa

Faailoga o oloa

E uiga i CYP:

Ituaiga tali fa'amamafa:

Fa'amatalaga o oloa:

Enzymes	Pusa su'esu'e (SynKit)	Fa'amatalaga
Enzyme Powder	ES-CYP-101~ ES-CYP-108	se seti o 8 Cytochrome P450 Monooxygenases, 50 mg taʻitasi 8 mea * 50mg / mea, poʻo isi aofaʻi
Pusa su'esu'e (SynKit)	ES-CYP-800	se seti o 8 Cytochrome P450 Monooxygenases, 1mg taʻitasi 8 mea * 1mg / mea

Tulaga lelei:

★ Alaleo ole substrate lautele.
★ Liliu maualuga.
★ Faʻaitiitia mea faʻatau.
★ Tulaga tali malie.
★ Siosiomaga.

Fa'atonuga mo le fa'aoga:

➢ E masani lava, o le faiga e tali atu ai e tatau ona aofia ai le mea'ai, vaifofo pa'u (O le tali sili ona lelei o le pH o le enzyme), coenzyme (NAD(H) po'o le NADP(H)), coenzyme regeneration system (eg glucose and glucose dehydrogenase) ma le ES-CYP.coenzyme ma le coenzyme regeneration system e mafai ona suia i le hydrogen peroxide ile vaega ole tali.
➢ O ituaiga uma o ES-CYPs e fetaui ma tulaga eseese o tali lelei e tatau ona su'esu'eina ta'ito'atasi.
➢ Fa'asa'o maualuga le mea'ai po'o se oloa e mafai ona fa'alavelaveina le gaioiga a le ES-CYP.Ae ui i lea, e mafai ona faʻamalolo le faʻalavelave e ala i le faʻaopoopoina o se vaega o substrate.

Fa'ata'ita'iga o Talosaga:

Faataitaiga 1⁽¹⁾:

Faataitaiga 2⁽²⁾:

Faataitaiga 3⁽³⁾:

Faataitaiga 4⁽⁴⁾:

Teuina:

Taofi le 2 tausaga i lalo -20℃.

Fa'alogo:

Aua ne'i fa'afeso'ota'i i tulaga ogaoga e pei o le: maualuga le vevela, maualuga/maualalo le pH ma le maualuga o le fa'aogaina o so'o.

Fa'asinomaga:

1. Zaretzki J, Matlock M, ma Swamidass S JJ Chem.Inf.Fa'ata'ita'iga, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez FA., e tal.J. Am.Chem.Soc., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic NJ., ma De Voss J J. Org.Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal.Biochim.Biophys.Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.;Cheon, WK e tal.Acta Crystallogr.2009, 65, 372.