Transaminase (ATA)
Enzymes: O macromolecular biological catalysts, o le tele o enzymes o polotini.
Transaminases: O se vasega o enzymes e faʻapipiʻi amino fesiitaiga i le va o amino acids ma keto acids.Transaminases o enzymes olaola autu i le synthesis asymmetric ma faʻataʻitaʻiga racemic o amines chiral.
Aminotransferase e mafai ona vaevaeina i ni vasega se fa e tusa ai ma le faasologa ma le fausaga: Ⅰ, Ⅱ, Ⅲ ma le Ⅳ.O ω-aminotransferases e aofia i le vasega II transaminases, e masani ona faʻaaogaina i le sauniuniga o amine chiral ma amino acids e le masani ai, e pei ole β-amino acids.
ω-aminotransferases: I le tele o tulaga, o le ω-transaminase e faasino i se vasega o enzymes, e faʻafeiloaʻi le ammonia catalytic e aunoa ma le α-amino acid e fai ma meaʻai poʻo oloa.
Catalytic masini:
| Enzymes | Code o oloa | Code o oloa |
| Enzyme Powder | ES-ATA-101~ ES-ATA-165 | se seti o 65 ω-Transaminases, 50 mg taʻitasi 65 mea * 50mg / mea, poʻo isi aofaʻi |
| Pusa su'esu'e (SynKit) | ES-ATA-6500 | se seti o 65 ω-Transaminases, 1 mg taitasi 65 aitema * 1mg / mea |
★ maualuga substrate specificity.
★ Malosi le filifilia o chiral.
★ Fa'aliliuga maualuga maualuga.
★ Faʻaitiitia mea faʻatau.
★ Tulaga tali malie.
★ Siosiomaga.
➢ E tatau ona faia le su'esu'ega o le enzyme mo mea'ai fa'apitoa ona o le tulaga fa'apitoa o le mea'ai, ma maua se enzyme e fa'agasolo ai le mea'ai fa'atatau i le fa'agata sili ona lelei.
➢ 'Aua ne'i fa'afeso'ota'i i tulaga ogaoga e pei o le: maualuga le vevela, maualuga/maualalo le pH ma so'o so'o fa'atasi ma le maualuga maualuga.
➢ E masani lava, o le faiga e tali atu ai e tatau ona aofia ai le substrate, vaifofo paʻu, foaʻi amino (e pei o amino acids ma le 1-phenyl ethylamine) poʻo le tali atu (e pei o keto acids), coenzyme (PLP), cosolvent (pei o le DMSO).
➢ E tatau ona fa'aopoopo mulimuli le ATA i le faiga fa'afoliga, pe a uma ona fetu'una'i le pH ma le vevela i le tulaga e tali atu ai.
➢ O ituaiga uma o ATA e iai tulaga e sili ona lelei e tali atu ai, o lea e tatau ai ona su'esu'eina ta'ito'atasi.
Fa'ata'ita'iga 1(fa'asologa o le Sitagliptin, fa'asologa fa'atusa)(1):
Fa'ata'ita'iga 2 (Mexiletine, Tu'ufa'atasiga o fa'ai'uga kinetic fa'atasi ai ma le fa'asologa fa'atusa)(2):
1 Savile CK, Janey JM, Mundorff EC, et al.Saienisi, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, et al.Tusi fa'aletino, 2009,11(21):4810-4812.
