Nitrilase (NIT)
Enzymes | Code o oloa | Fa'amatalaga |
Enzyme Powder | ES-NIT-101~ ES-NIT-140 | se seti o le 40 Nitrilase, 50 mg taʻitasi 40 mea * 50mg / mea, poʻo isi aofaʻi |
Pusa su'esu'e (SynKit) | ES-NIT-4000 | se seti o 40 Nitrilase, 1 mg taitasi 40 mea * 1mg / mea |
★ maualuga substrate specificity.
★ Liliu maualuga.
★ Faʻaitiitia mea faʻatau.
★ Tulaga tali malie.
★ Siosiomaga.
➢ E masani lava, o le faiga e tali atu ai e tatau ona aofia ai le mea'ai, vaifofo pa'u (pH tali sili ona lelei) ma le ES-NIT.
➢ E mafai ona fa'ata'ita'i uma ES-NIT i le faiga o tali i luga po'o le NIT Screening Kit (SynKit NIT).
➢ O ituaiga uma o ES-NIT e fetaui ma tulaga eseese o tali sili ona lelei e tatau ona su'esu'eina ta'ito'atasi.
➢ Fa'asa'o maualuga le mea'ai po'o se oloa e mafai ona fa'alavelaveina le gaioiga a le ES-NIT.Ae ui i lea, e mafai ona faʻamalolo le faʻalavelave e ala i le faʻaopoopoina o se vaega o substrate.
Fa'ata'ita'iga 1(Fa'agasolo ole nicotinic acid)(1):
Fa'ata'ita'iga 2(Fa'agasolo ole (R)-(-)-mandelic acid)(2):
Fa'ata'ita'iga 3(Fa'agasolo ole baclofen)(3):
Fa'ata'ita'iga 4(Fa'agasolo ole glycolic acid)(4):
Taofi le 2 tausaga i lalo -20℃.
Aua ne'i fa'afeso'ota'i i tulaga ogaoga e pei o le: maualuga le vevela, maualuga/maualalo le pH ma le maualuga o le fa'aogaina o so'o.
1 Mathew CD, Nagasawa T, Kobayashi M, e tal.Appl.Si'osi'omaga.Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal.Appl.Si'osi'omaga.Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, e tal.Chin.J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, e tal.Biotechnol.Bioeng., 2008, 99(3): 717.