Nitrile reductase (NRED)
Fa'amatalaga Puupuu:
E uiga i le Nitrile reductase
E 3 ituaiga NRED enzyme oloa (Numera pei ES-NRED101~ ES-NRED103) atiaʻe e SyncoZymes.E mafai e le NRED ona faʻamalosia le faʻaitiitia o le vaega nitrile i amine.I le faagasologa o le catalysis, e manaʻomia le NADPH e avea ma felauaiga hydrogen.
Nitrile reductasecatalytic reaction type
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I-Meli:lchen@syncozymes.com
| Enzymes | Code o oloa | Fa'amatalaga |
| Fa'amatalaga | ES- NRED -101~ ES- NRED -103 | se seti o le 3 Nitrile Reductase, 50 mg taʻitasi 3 mea * 50mg / mea, poʻo isi aofaʻi |
E mafai ona fa'aoga le faiga fa'afoliga lea e fa'asino i ai:
1-10 mg/ml Fa'apalapala
10 mg/ml Enzyme
10% (v/v) Sosoo fa'aletino (1)
100 mM Fofosa fa'apipi'i (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Kulukose
2 mg/ml Glucose dehydrogenase (GDH)
Fa'ato'a fa'a'ai i se fa'amalo vevela faifai pea (fa'ata'ita'iga, 30oC, 150rpm) i totonu mo le 24~48 itula.Centrifuge paluga taitasi e precipitate porotini, latou le supernatant e mafai ona faaaoga mo le TLC, HPLC po o le GC suesuega.
Fa'aaliga (1): Soli fa'aletino (pei o le DMSO, methanol, acetonitrile, IPA, ma isi) e filifili, fa'aaoga mo mea e le mafai ona fa'aaogaina.
