Leucine Dehydrogenase (LeuDH)
Fa'amatalaga Puupuu:
E uiga i le Leucine dehydrogenase
E 1 le ituaiga o oloa enzyme LeuDH (Numera e pei o le ES-LeuDH-101) na atiaʻe e SyncoZymes.LeuDH e mafai ona faʻamalosia le faʻaitiitia o le ammoniation o le 4-methylbutanoic acid (masima) i le L-leucine poʻo le faʻamaʻiina o le leucine i le 4-methylbutanoic acid.E mafai foi e le ES-LeuDH ona faʻapipiʻiina le faʻaitiitia o le amination o le α-keto acids faʻatasi ai ma le fausaga tutusa e saunia ai mea e maua mai i le amino acid.I le faagasologa o le catalysis, e manaʻomia le NADH e pei o le hydrogen transporter.
Leucine dehydrogenasecatalytic reaction type
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I-Meli:lchen@syncozymes.com
| Enzymes | Code o oloa | Fa'amatalaga |
| Enzyme Powder | ES-LeuDH-101 | se seti o le 1 Monoamine Oxidase, 50 mg taʻitasi 1 mea * 50mg / mea, poʻo isi aofaʻi |
E mafai ona fa'aoga le faiga fa'afoliga lea e fa'asino i ai:
1-10 mg/ml: Fa'asa'o
10 mg/ml: Enzyme
10% (v/v): Sosoo fa'aletino (1)
100 mM: Fa'asao fa'afefete (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Fa'asologa o le dehydrogenase (FDH)
Fa'ato'a fa'a'ai i se fa'amalo vevela faifai pea (fa'ata'ita'iga, 30oC, 150rpm) i totonu mo le 24~48 itula.Centrifuge paluga taʻitasi e faʻafefe ai porotini, e mafai ona faʻaaogaina le supernatant mo suʻesuʻega TLC, HPLC poʻo le GC.
Fa'aaliga (1): Soli fa'aletino (pei o le DMSO, methanol, acetonitrile, IPA, ma isi) e filifili, fa'aaoga mo mea e le mafai ona fa'aaogaina.
