Lactate Dehydrogenase (LDH)
Fa'amatalaga Puupuu:
E uiga i le Lactate dehydrogenase
E 1 le ituaiga LDH enzyme product (Numera pei ES-LDH) na atiaʻe e SyncoZymes.E mafai e le LDH ona fa'atupuina le fa'aitiitiga o le pyruvate i le L-lactic acid, po'o le fa'aitiitia o isi vaila'au α-keto tutusa.I le faagasologa o le catalysis, e manaʻomia le NADH e pei o le hydrogen transporter.
Lactate dehydrogenasecatalytic ituaiga tali
Mobile/Wechat/WhatsApp: +86-13681683526
I-Meli:lchen@syncozymes.com
| Enzymes | Code o oloa | Fa'amatalaga |
| Enzyme Powder | ES-LDH-101 | se seti o le 1 Monoamine Oxidase, 50 mg taʻitasi 1 mea * 50mg / mea, poʻo isi aofaʻi |
E mafai ona fa'aoga le faiga fa'afoliga lea e fa'asino i ai:
1-10 mg/ml: Fa'asa'o
10 mg/ml: Enzyme
10% (v/v): Sosoo fa'aletino (1)
100 mM: Fa'asao fa'afefete (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Fa'asologa o le dehydrogenase (FDH)
Fa'ato'a fa'a'ai i se fa'amalo vevela faifai pea (fa'ata'ita'iga, 30oC, 150rpm) i totonu mo le 24~48 itula.Centrifuge paluga taʻitasi e faʻafefe ai porotini, e mafai ona faʻaaogaina le supernatant mo suʻesuʻega TLC, HPLC poʻo le GC.
Fa'aaliga (1): Soli fa'aletino (pei o le DMSO, methanol, acetonitrile, IPA, ma isi) e filifili, fa'aaoga mo mea e le mafai ona fa'aaogaina.
