Ua le mafai ona gaoioi CALB
O le CALB e le mafai ona gaoioi e ala i le adsorption faaletino i luga o le resin hydrophobic maualuga o le macroporous, styrene/methacrylate polymer.Immobilized CALB e fetaui lelei mo faʻaoga i soʻo faʻalagona ma faiga e leai se solvent, ma e mafai ona toe faʻaaogaina ma toe faʻaaogaina mo le tele o taimi i tulaga talafeagai.
Code o oloa: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.
★Gaioioiga maualuga, filifilia chiral maualuga ma mautu maualuga.
★Le lelei le faatinoga i vaega e le o le vai.
★ Fa'afaigofie ona aveese mai le faiga o tali, vave fa'amutaina tali, ma aloese mai mea o totoe o le polotini i oloa.
★E mafai ona toe faʻaaogaina ma toe faʻaoga e faʻaitiitia ai le tau.
| Gaoioiga | ≥10000PLU/g |
| pH laina mo tali | 5-9 |
| Va'aiga vevela mo tali | 10-60 ℃ |
| Fa'aaliga | CALB-IMMO100-A: Samasama mumu i le lanu enaena malo CALB-IMMO100-B: Pa'epa'e i le lanu enaena mumu |
| Lapopo'a o vaega | 300-500μm |
| Ma'imau ile fa'amago ile 105℃ | 0.5%-3.0% |
| Resin mo le le mafai ona gaoioi | Macroporous, styrene/metacrylate polymer |
| Soli fa'afefe | Suavai, solvent organic, ma isi, pe leai foi se solvent.Mo le tali atu i nisi solvents organic, 3% vai e mafai ona faaopoopo e faaleleia le aafiaga tali |
| Lapopo'a o vaega | CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm |
Fa'amatalaga o le iunite: 1 le iunite e fetaui ma le tu'ufa'atasiga o le 1μmol i le minute propyl laurate mai lauric acid ma le 1-propanol i le 60 ℃.O loʻo i luga CALB-IMMP100-A ma CALB-IMMO100-B e fetaui ma vaʻavaʻavaʻavaʻavaʻa faʻatasi ma lapopoʻa eseese.
1. Ituaiga reactor
O le enzyme immobilized e fa'aoga uma i le kettle batch reactor ma le moega fa'amautu fa'asolo fa'aauau.E tatau ona maitauina e aloese mai le nutimomoia ona o le malosi mai fafo i le taimi o le fafagaina poʻo le faʻatumu.
2. Tali pH, vevela ma solvent
E tatau ona fa'aopoopo le enzyme immobilized mulimuli, pe a uma ona fa'aopoopo ma fa'amavae isi mea, ma fetuutuunai le pH.
Afai o le faʻaaogaina o le substrate poʻo le faʻavaeina o le oloa o le a taʻitaʻia ai le suiga o le pH i le taimi o le tali, e tatau ona lava le paʻu e faʻaopoopo i le faiga o tali, poʻo le pH e tatau ona mataʻituina ma fetuunai i le taimi o le tali.
I totonu ole lavelave faapalepale ole vevela ole CALB (i lalo ifo o le 60 ℃), o le fua faatatau o le liua na faʻateleina i le faʻateleina o le vevela.I le faʻaaogaina masani, e tatau ona filifilia le vevela o le tali e tusa ai ma le mautu o le substrate poʻo le oloa.
E masani lava, o le ester hydrolysis tali e talafeagai i le aqueous vaega faiga, ae o le ester synthesis tali e talafeagai i le faiga vaega vaega.O le solvent organic e mafai ona avea ma ethanol, tetrahydrofuran, n-hexane, n-heptane ma toluene, poʻo se solvent fefiloi talafeagai.Mo le tali atu i nisi solvents organic, 3% vai e mafai ona faaopoopo e faaleleia le aafiaga tali.
3. Toe fa'aaogaina ma le ola tautua o le CALB
I lalo o le tulaga talafeagai tali, e mafai ona toe faʻaleleia ma toe faʻaaogaina CALB, ma o taimi faʻapitoa e faʻaoga ai e eseese ma galuega eseese.
Afai e le toe fa'aaogaina le CALB toe fa'aleleia ma e mana'omia ona teu pe a uma ona toe fa'aleleia, e mana'omia ona fufulu ma fa'agogo ma fa'amaufa'ailoga ile 2-8 ℃.
A mae'a le tele o ta'amilosaga o le toe fa'aoga, pe a fa'aitiitia teisi le lelei o le tali, e mafai ona fa'aopoopo le CALB ma fa'aauau ona fa'aoga.Afai e matua fa'aitiitia le lelei o le tali, e tatau ona sui.
Faataitaiga 1(Aminolysis)(1):
Faataitaiga 2(Aminolysis)(2):
Fa'ata'ita'iga 3(Fa'amama le fa'aputuina o polyester)(3):
Faataitaiga 4(Transesterification, regioselective of hydroxyl group)(4):
Faataitaiga 5(Transesterification, fa'ato'a fa'ateteleina o 'ava mālosi)(5):
Fa'ata'ita'iga 6(Esterification, kinetic resolution of carboxylic acid)(6):
Faataitaiga 7(Esterolysis, kinetic resolution)(7):
Faataitaiga 8(Hydrolysis of amides)(8):
Faataitaiga 9(Acylation of amines)(9):
Fa'ata'ita'iga 10(Aza-Michael fa'aopoopo tali)(10):
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