Leucine Dehydrogenase (LeuDH)
Fa'amatalaga Pupuu:
E uiga i le Leucine dehydrogenase
E tasi le ituaiga oloa o le enzyme LeuDH (Numera o le ES-LeuDH-101) na atiae e SyncoZymes. E mafai e le LeuDH ona fa'agaoioia le fa'aitiitia o le ammoniation o le 4-methylbutanoic acid (masima) i le L-leucine po'o le fa'a'okeseneina o le leucine i le 4-methylbutanoic acid. E mafai fo'i e le ES-LeuDH ona fa'agaoioia le fa'aitiitia o le amination o α-keto acids ma le fausaga tutusa e saunia ai mea e maua mai i amino acid. I le faagasologa o le catalysis, e mana'omia le NADH e fai ma aveta'avale hydrogen.
Leucine dehydrogenase ituaiga o tali fa'acatalytic
Telefoni feavea'i/Wechat/WhatsApp: +86-13681683526
Imeli:lchen@syncozymes.com
| Enzima | Fa'ailoga o le Oloa | Fa'amatalaga |
| Pauta Enzyme | ES-LeuDH-101 | o se seti o le 1 Monoamine Oxidase, 50 mg i le seti 1 mea * 50mg / mea, po'o se isi lava aofa'i |
E mafai ona fa'aaogaina le faiga o tali atu lea mo se fa'asinomaga:
1-10 mg/ml: Mea e fa'aputu ai
10 mg/ml: Enzyme
10% (v/v): Fa'aola fa'aolaola (1)
100 mM: Fa'apa'u phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Fa'ama'i o le Formate dehydrogenase (FDH)
Fa'apuna ia tali i se masini lūlū e tumau le vevela (mo se fa'ata'ita'iga, 30oC, 150rpm) mo le 24~48 itula. Fa'asolo i totonu o le centrifuge paluga ta'itasi e fa'aputu ai porotini, ona mafai lea ona fa'aogaina le supernatant mo le TLC, HPLC po'o le au'ili'iliga GC.
Fa'amatalaga (1): O le vaila'au fa'aola (e pei o le DMSO, methanol, acetonitrile, IPA, ma isi) e le mana'omia, e fa'aaogaina mo mea e le mafai ona solo.
