Lactate Dehydrogenase (LDH)
Fa'amatalaga Pupuu:
E uiga i le Lactate dehydrogenase
E tasi le ituaiga oloa o le enzyme LDH (Numera o le ES-LDH) na atiae e SyncoZymes. E mafai e le LDH ona fa'aitiitia le pyruvate i le L-lactic acid, po'o le fa'aitiitia o isi α-keto acids tutusa. I le faagasologa o le fa'asolosolo, e mana'omia le NADH e fai ma aveta'avale o le hydrogen.
Ituaiga o tali fa'acatalytic lactate dehydrogenase
Telefoni feavea'i/Wechat/WhatsApp: +86-13681683526
Imeli:lchen@syncozymes.com
| Enzima | Fa'ailoga o le Oloa | Fa'amatalaga |
| Pauta Enzyme | ES-LDH-101 | o se seti o le 1 Monoamine Oxidase, 50 mg i le seti 1 mea * 50mg / mea, po'o se isi lava aofa'i |
E mafai ona fa'aaogaina le faiga o tali atu lea mo se fa'asinomaga:
1-10 mg/ml: Mea e fa'aputu ai
10 mg/ml: Enzyme
10% (v/v): Fa'aola fa'aolaola (1)
100 mM: Fa'apa'u phosphate (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Fa'ama'i o le Formate dehydrogenase (FDH)
Fa'apuna ia tali i se masini lūlū e tumau le vevela (mo se fa'ata'ita'iga, 30oC, 150rpm) mo le 24~48 itula. Fa'asolo i totonu o le centrifuge paluga ta'itasi e fa'aputu ai porotini, ona mafai lea ona fa'aogaina le supernatant mo le TLC, HPLC po'o le au'ili'iliga GC.
Fa'amatalaga (1): O le vaila'au fa'aola (e pei o le DMSO, methanol, acetonitrile, IPA, ma isi) e le mana'omia, e fa'aaogaina mo mea e le mafai ona solo.
