Hydroaminase (HAM)
Fa'amatalaga Puupuu:
E uiga i le Hydroaminase
E 2 ituaiga o oloa enzyme HAM (Numera e pei ES-HAM-101~ ES-HAM-102) atiaʻe e SyncoZymes.E mafai e le HAM ona fa'atupuina le ammoniation o le enoic acid po'o ona mea e maua mai ai le chiral amino acids.E mafai ona fa'aogaina le HAM e fa'atupu ai le chiral amino acids (po'o chiral amines) mai enoic acids (po'o alkenes).Ammonia foaʻi, e pei o le vai ammonia poʻo le masima ammonium, e manaʻomia.
Hydroaminase catalytic ituaiga tali
Mobile/Wechat/WhatsApp: +86-13681683526
I-Meli:lchen@syncozymes.com
| Enzymes | Code o oloa | Fa'amatalaga |
| Enzyme Powder | ES-HAM-101~ ES-HAM-102 | se seti o 2 Nuclosidephosphoryalse, 50 mg taʻitasi 2 mea * 50mg / mea, poʻo isi aofaʻi |
E mafai ona fa'aoga le faiga fa'afoliga lea e fa'asino i ai:
1-10 mg/ml: Fa'asa'o
10 mg/ml: Enzyme
10% (v/v): Sosoo fa'aletino (1)
10% (v/v): Amonia
Fetuuna'i le pH i le 9.0 a'o le'i fa'aopoopoina le enzyme, ma e tatau ona fa'aopoopo mulimuli ane le enzyme.Fa'ato'a fa'a'ai i se fa'amalo vevela faifai pea (fa'ata'ita'iga, 30oC, 150rpm) i totonu mo le 24~48 itula.Centrifuge paluga taitasi e precipitate porotini, latou le supernatant e mafai ona faaaoga mo le TLC, HPLC po o le GC suesuega.
Fa'aaliga (1): Soli fa'aletino (pei o le DMSO, methanol, acetonitrile, IPA, ma isi) e filifili, fa'aaoga mo mea e le mafai ona fa'aaogaina.
