Epoxide hydrolase (EH)
Fa'amatalaga Pupuu:
E uiga i le epoxide hydrolase
O le EH o le ether hydrolase lea e fa'atino ai le stereoselective hydrolysis o racemic epoxides e gaosia ai le 1, 2-diol ma le optically active epoxides, lea e mafai ona fa'aaogaina mo le chiral resolution o epoxides po'o le sauniaina o chiral diols. E fa'aaogaina lautele i vaila'au ma isi matā'upu. E 3 ituaiga o oloa epoxide hydrolases (Numera o le ES-EH-101～ES-EH-103) na atia'e e SyncoZymes. O le ES-EH o se meafaigaluega aoga e fa'atino ai le synthesis o chiral alcohols e ala i le stereoselective hydrolysis o aliphatic ma aromatic epoxides.
Ituaiga o tali fa'a-catalytic:
Telefoni feavea'i/Wechat/WhatsApp: +86-13681683526
Imeli:lchen@syncozymes.com
| Enzima | Fa'ailoga o le Oloa | Fa'amatalaga |
| Pauta Enzyme | ES-EH-101~ ES-EH-103 | o se seti o Epoxide Hydrolases e 3, 50 mg i le seti 3 mea * 50mg / mea, po'o se isi lava aofa'i |
| Meafaigaluega mo le Su'esu'ega (SynKit) | ES-EH-300 | o se seti o Epoxide Hydrolases e 3, 1 mg i mea ta'itasi 3 * 1mg / mea |
★ Maualuga le tulaga fa'apitoa o le substrate.
★ Filifiliga malosi o le chiral.
★ Liuga maualuga.
★ E itiiti ifo oloa e maua mai ai.
★ Tulaga o le tali atu e le ogaoga.
★ Fa'alelei i le siosiomaga.
➢ E masani lava, e tatau ona aofia i le faiga o le tali atu le substrate, le buffer solution (pH sili ona lelei o le tali atu) ma le ES-EH.
➢ E tatau ona suʻesuʻeina taʻitasi ituaiga uma o ES-EH e fetaui ma tulaga eseese o le tali atu sili ona lelei.
➢ O le substrate po'o le oloa e maualuga le malosi e ono taofia ai le gaioiga a le ES-EH. Peita'i, e mafai ona fa'aitiitia le fa'alavelave e ala i le fa'aopoopoina o le substrate i se vaega tele.
Faʻataʻitaʻiga 1(1):
Tausi mo le 2 tausaga i lalo ifo o le -20℃.
Aua lava ne'i pa'i atu i ni tulaga ogaoga e pei o le: vevela maualuga, pH maualuga/maualalo ma le vaila'au fa'aola e maualuga le malosi.
1. Kim YH, Lee I, Choi SH, e tal. J Mol Catal B-Enzym, 2013, 89: 48-51.
