Amidase (AMD)
Enzymes:O macromolecular biological catalysts, o le tele o enzymes o polotini
Amidase:Fa'asalaina le fa'asusuina o aliphatic ma mea manogi mai fafo e ala i le tu'uina atu o se vaega acyl i vai fa'atasi ai ma le gaosiga o ga'o saoloto ma ammonia.Hydroxamic acids ma isi organic acids e masani ona faʻaaogaina e fai ma vailaʻau aua o loʻo iai i latou o mea e tupu aʻe ai, vailaʻau faʻamaʻi ma faʻalavelave tumo.E mafai ona vaevaeina amidases i ituaiga R ma S ituaiga acylases e tusa ai ma le stereoselectivity catalyst.
I le faaopoopo atu i le faʻapipiʻiina o le hydrolysis o amides, e mafai foi e le amidase ona faʻafefeina faʻaliliuga acyl i le i ai o mea faʻapipiʻi e pei ole hydroxylamine.
Amidase ma punaoa eseese e eseese substrate specificity, o nisi oi latou e mafai na o hydrolyze aromatic amides, o nisi oi latou e na o le hydrolyze aliphatic amides, ma nisi hydrolyze α-po o ω-amino amides.O le tele o amine e lelei le gaioioiga mo na'o acyclic po'o faigofie aromatic amides, ae mo mea manogi lavelave, heterocyclic amides, ae maise amides faatasi ai ma ortho substituents, e masani lava ona maualalo le gaioiga (naʻo ni nai enzyme e faʻaalia ai le sili atu o aʻafiaga catalytic).
Catalytic masini:
| Enzymes | Code o oloa | Code o oloa |
| Enzyme Powder | ES-AMD-101~ ES-AMD-119 | se seti o 19 amidases, 50 mg taʻitasi 19 mea * 50mg / mea, poʻo isi aofaʻi |
| Pusa su'esu'e (SynKit) | ES-AMD-1900 | se seti o 19 amidases, 1 mg taitasi 19 mea * 1mg / mea |
★ maualuga substrate specificity.
★ Malosi le filifilia o chiral.
★ Fa'aliliuga maualuga maualuga.
★ Faʻaitiitia mea faʻatau.
★ Tulaga tali malie.
★ Siosiomaga.
➢ E tatau ona faia le su'esu'ega o le enzyme mo mea'ai fa'apitoa ona o le tulaga fa'apitoa o le mea'ai, ma maua se enzyme e fa'agasolo ai le mea'ai fa'atatau i le fa'agata sili ona lelei.
➢ 'Aua ne'i fa'afeso'ota'i i tulaga ogaoga e pei o le: maualuga le vevela, maualuga/maualalo le pH ma so'o so'o fa'atasi ma le maualuga maualuga.
➢ E masani lava, o le faiga e tali atu ai e tatau ona aofia ai le mea'ai, vaifofo pa'u (O le pH tali sili ona lelei o le enzyme).E tatau ona iai fa'atasi fa'atasi e pei o le hydroxylamine i le faiga fa'aliliuga acyl.
➢ E tatau ona toe fa'aopoopo le AMD i le faiga fa'afoliga fa'atasi ai ma le maualuga o tali pH ma le vevela.
➢ O ituaiga AMD uma lava e iai tulaga e sili ona lelei e tali atu ai, o lea e tatau ai ona su'esu'eina ta'ito'atasi.
Faataitaiga 1(1):
Gaoioiga hydrolysis i mea eseese Amide Substrates
| Alafua | Gaoioiga faapitoa μmols min-1mg-1 | Alafua | Gaoioiga faapitoa μmols min-1mg-1 |
| Acetamide | 3.8 | ο-OH benzamide | 1.4 |
| Propionamide | 3.9 | p-OH benzamide | 1.2 |
| Lactamide | 12.8 | ο-NH2benzamide | 1.0 |
| Butyramide | 11.9 | p-NH2benzamide | 0.8 |
| Isobutyramide | 26.2 | ο-Toluamide | 0.3 |
| Pentanamide | 22.0 | p-Toluamide | 8.1 |
| Hexanamide | 6.4 | Nicotinamide | 1.7 |
| Cyclohexanamide | 19.5 | Isonicotinamide | 1.8 |
| Acrylamide | 10.2 | Picolinamide | 2.1 |
| Metacrylamide | 3.5 | 3-Phenylpropionamide | 7.6 |
| Prolinamide | 3.4 | Indol-3-acetamide | 1.9 |
| Benzamide | 6.8 |
O le tali na faia i le 50mM sodium phosphate buffer solution, pH 7.5, ile 70 ℃.
| Amides | Hydroxylamine | Hydrazine |
| Acetamide | 8.4 | 1.4 |
| Propionamide | 18.4 | 3.0 |
| Isobutyramide | 25.0 | 22.7 |
| Benzamide | 9.2 | 6.1 |
O le tali na faia i le 50mM sodium phosphate buffer solution, pH 7.5, ile 70 ℃.
Fa'atatauga fa'a'au'au: amides, 100 mM(benzamide, 10 mM);hydroxylamine ma hydrazine, 400 mM;enzyme 0.9 μM.
Faataitaiga 2(2):
Faataitaiga 3(3):
1. D'Abusco AS, Ammendola S., et al.Extremophiles, 2001, 5:183-192.
2. Guo FM, Wu JP, Yang LR, et al.Fa'agasologa Biochemistry, 2015, 50(8): 1400-1404.
3. Zheng RC, Jin JQ, Wu ZM, et al.Bioorganic Chemistry, 2017, Avanoa i luga ole laiga 7.
