Phenylalanine ammonia lyase (PAL)
Fa'amatalaga Pupuu:
E uiga i le Phenylalanine ammonia lyase
ES-PAL: O se vasega o enzymes e fa'atino ai le fa'amamāina tuusa'o o le L-phenylalanine i le trans-cinnamic acid. Na atia'e e Syncozymes ni mea e 10 o le phenylalanine ammonia lyase (fa'anumera ES-PAL-101~ES-PAL-110), lea e mafai ona fa'aaogaina i le fa'amamāina po'o le fa'afeagai o le tali atu o le phenylalanine ma ona mea e maua mai ai.
Ituaiga o tali fa'a-catalytic:
Telefoni feavea'i/Wechat/WhatsApp: +86-13681683526
Imeli:lchen@syncozymes.com
| Enzima | Fa'ailoga o le Oloa | Fa'amatalaga |
| Pauta Enzyme | ES-PAL-101~ ES-PAL-110 | o se seti o le 10 L-phenylalanine ammonia-lyase, 50 mg i mea ta'itasi 10 * 50mg / mea, po'o se isi aofa'i |
★ Maualuga le tulaga fa'apitoa o le substrate.
★ Filifiliga malosi o le chiral.
★ Liuga maualuga.
★ E itiiti ifo oloa e maua mai ai.
★ Tulaga o le tali atu e le ogaoga.
★ Fa'alelei i le siosiomaga.
➢ E masani lava, e tatau ona aofia i le faiga o tali le substrate, le buffer solution ma le enzyme.
➢ O ituaiga taʻitasi o le ES-PAL e fetaui ma tulaga eseese o le tali atu sili ona lelei, lea e mafai ona suʻesuʻeina taʻitasi.
➢ E tatau ona fa'aopoopo mulimuli le ES-PAL i totonu o le faiga o tali atu ina ia fa'atumauina ai le gaioiga.
Faʻataʻitaʻiga 1 (Faʻasologa o le trans cinnamic acid mai le phenylalanine)(1):
Faʻataʻitaʻiga 2 (Faʻasologa o le phenylalanine derivative mai le trans-cinnamic acid derivative)(2):
Tausi mo le 2 tausaga i lalo ifo o le -20℃.
Aua lava ne'i pa'i atu i ni tulaga ogaoga e pei o le: vevela maualuga, pH maualuga/maualalo ma le vaila'au fa'aola e maualuga le malosi.
1. Zachary Mays, Karishma Mohan,ma isi.Chem Commun (Camb).2020, 56, 5255-5258.
2. Ian Rowles, Bas Groenendaal,ma isi. Tetrahedron,2016. 72, 7343-7347.
