Nucloside phosphoryalse (NP)

Fa'amatalaga Puupuu:

E uiga i le nucleoside phosphoryalse

E 3 ituaiga o oloa enzyme NP (Numera pei ES-NP-101~ ES-NP-103) atiaʻe e SyncoZymes.ES-NP-101 o le purine nucleoside phosphorylase, ES-NP-102 ma le ES-NP-103 o le pyrimidine nucleoside phosphorylase.Nucleosidephosphorylase e mafai ona pala le nucleosides i faavae ma pentose phosphate.Nucleoside phosphorylase e mafai ona vaevaeina i le purine nucleoside phosphorylase ma le pyrimidine nucleoside phosphorylase e tusa ai ma le manaʻoga o faʻavae nucleoside.O le purine nucleoside phosphorylase e mafai ona faʻaaogaina le adenosine i le adenine, inosine i le hypoxanthine, guanosine i le guanine, ma maua ai le ribose phosphate i le taimi e tasi.Pyrimidine nucleoside phosphorylase e mafai ona faʻafefete uridine i le uracil ma maua ai le ribose phosphate.

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Fa'amatalaga Oloa

Faailoga o oloa

Nucloside phosphoryalse ituaiga tali:

Fa'amatalaga o oloa:

Enzymes	Code o oloa	Fa'amatalaga
Enzyme Powder	ES-NP-101~ ES-NP-103	se seti o 3 Nuclosidephosphoryalse, 50 mg taʻitasi 3 mea * 50mg / mea, poʻo isi aofaʻi

Nucloside phosphoryalse su'esu'ega faiga:

E mafai ona fa'aoga le faiga fa'afoliga lea e fa'asino i ai:
1-10 mg/ml Fa'apalapala
10 mg/ml Enzyme
100 mM Fofosa fa'apipi'i (pH7.0)
Fa'ato'a fa'a'ai i se fa'amalo vevela faifai pea (mo se fa'ata'ita'iga, 30oC, 150rpm) i totonu mo le 12~24 itula.Centrifuge paluga taʻitasi e faʻafefe ai porotini, e mafai ona faʻaaogaina le supernatant mo suʻesuʻega TLC, HPLC poʻo le GC.