Nitrile hydratase (NHT)
Fa'amatalaga Puupuu:
E uiga i ES-NHTs
ES-NHTs: o se vasega o metalloenzyme, lea e filifilia faʻamaʻaina nitriles i totonu ole amide muamua tutusa.E faʻaaogaina lautele e le gata mo le tuʻufaʻatasia o amino acids, amides, carboxylic acids ma a latou mea e maua mai ai, ae faʻapea foʻi mo le sauniuniga o vailaʻau chiral.E 24 ituaiga o nitrile hydratases na atia'e e SyncoZymes (Numera pei ES-NHT-101～ES-NHT-124).
Ituaiga tali fa'amamafa:
Mobile/Wechat/WhatsApp: +86-13681683526
I-Meli:lchen@syncozymes.com
| Enzymes | Code o oloa | Fa'amatalaga |
| Enzyme Powder | ES-NHT-101~ ES-NHT-124 | se seti o 24 Nitrile Hydratases, 50 mg taitasi 24 mea * 50mg / mea, poʻo isi aofaʻi |
| Pusa su'esu'e (SynKit) | ES-NHT-2400 | se seti o 24 Nnitrile Hydratases, 1 mg taitasi 24 aitema * 1mg / mea |
★ maualuga substrate specificity.
★ Malosi le filifilia o chiral.
★ Liliu maualuga.
★ Faʻaitiitia mea faʻatau.
★ Tulaga tali malie.
★ Siosiomaga.
➢ E masani lava, o le faiga e tali atu ai e tatau ona aofia ai le mea'ai, vaifofo pa'u (pH tali sili ona lelei) ma le ES-NHT.
➢ E mafai ona fa'ata'ita'i uma ES-NHT i le faiga fa'afoliga o lo'o i luga po'o le NHT Screening Kit (SynKit NHT).
➢ O ituaiga uma o ES-NHTs e fetaui ma tulaga eseese o tali sili ona lelei e tatau ona su'esu'eina ta'ito'atasi.
➢ Fa'asa'o maualuga le mea'ai po'o se oloa e mafai ona fa'alavelaveina le gaioiga a le ES-NHT.Ae ui i lea, e mafai ona faʻamalolo le faʻalavelave e ala i le faʻaopoopoina o mea faʻapipiʻi, faʻatasi ma amidases poʻo le faʻaaogaina o oloa.
Fa'ata'ita'iga 1(fa'atasi ma le amidase)(1):
Faataitaiga 2(2):
Taofi le 2 tausaga i lalo -20℃.
Aua ne'i fa'afeso'ota'i i tulaga ogaoga e pei o le: maualuga le vevela, maualuga/maualalo le pH ma le maualuga o le fa'aogaina o so'o.
1 Vojtech V, Ludmila M, Alicja BV, e tal.J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, e tal.Tetrahedron lett, 2010, 51: 1639-1641.
