Phenylalanine dehydrogenase (PDH)
Fa'amatalaga Pupuu:
E uiga i le Phenylalanine dehydrogenase
ES-PDH: O se vasega o le oxidoreductase. O le tali lelei e mafai ona fa'agaoioia ai le fa'ama'a'aina o le oxidative o le L phenylalanine salt i le i ai o le NAD e gaosia ai le phenylpyruvate salt, ma o le tali fa'afeagai e mafai ona fa'agaoioia ai le fa'aputuina o le amino acid salt mai le phenylpyruvate salt. Na atia'e e Syncozymes ni vaega e 8 o le phenylalanine dehydrogenase (fa'anumera ES-PDH-101~ES-PDH-108). E mafai ona fa'aaoga le ES-PDH e saunia ai le phenylalanine salt po'o le amino acid salt mai le phenylpyruvic acid salt po'o ni mea e maua mai ai.
Ituaiga o tali fa'a-catalytic:
Telefoni feavea'i/Wechat/WhatsApp: +86-13681683526
Imeli:lchen@syncozymes.com
| Enzima | Fa'ailoga o le Oloa | Fa'amatalaga |
| Pauta Enzyme | ES-PDH-101~ ES-PDH-108 | o se seti o le 8 Nitrile Reductase, 50 mg ta'itasi 8 mea * 50mg / mea, po'o se isi lava aofa'i |
★ Maualuga le tulaga fa'apitoa o le substrate.
★ Filifiliga malosi o le chiral.
★ Liuga maualuga.
★ E itiiti ifo oloa e maua mai ai.
★ Tulaga o le tali atu e le ogaoga.
★ Fa'alelei i le siosiomaga.
➢ I le tulaga masani, e tatau ona aofia i le faiga o le tali atu le substrate, buffer solution, enzyme, coenzyme (NAD(H)), coenzyme regeneration system (e.g. ammonium formate ma formate dehydrogenase).
➢ O ituaiga taʻitasi o ES-PDH e fetaui ma tulaga eseese o le tali atu, lea e mafai ona suʻesuʻeina taʻitasi pe a manaʻomia.
➢ E tatau ona fa'aopoopo mulimuli le ES-PDH i totonu o le faiga o tali atu ina ia fa'atumauina ai le gaioiga.
Faʻataʻitaʻiga 1 (Faʻasologa o mea e maua mai i le phenylalanine mai mea e maua mai i le phenylpyruvate)(1):
Tausi mo le 2 tausaga i lalo ifo o le -20℃.
Aua lava ne'i pa'i atu i ni tulaga ogaoga e pei o le: vevela maualuga, pH maualuga/maualalo ma le vaila'au fa'aola e maualuga le malosi.
1. Francesca Paradisi, Stuart Collins, ma isi. Journal of Biotechnology, 2007, 128, 408–411.
