Phenylalanine dehydrogenase (PDH)
Enzymes | Code o oloa | Fa'amatalaga |
Enzyme Powder | ES-PDH-101~ ES-PDH-108 | se seti o le 8 Nitrile Reductase, 50 mg taʻitasi 8 mea * 50mg / mea, poʻo isi aofaʻi |
★ maualuga substrate specificity.
★ Malosi le filifilia o chiral.
★ Liliu maualuga.
★ Faʻaitiitia mea faʻatau.
★ Tulaga tali malie.
★ Siosiomaga.
➢ E masani lava, o le faiga e tali atu ai e tatau ona aofia ai le substrate, vaifofo pa'u, enzyme, coenzyme (NAD(H)), coenzyme regeneration system (eg ammonium formate ma formate dehydrogenase).
➢ O ituaiga ES-PDH ta'itasi e fetaui ma tulaga eseese o tali lelei, e mafai ona su'esu'eina ta'ito'atasi pe a mana'omia.
➢ Ole ES-PDH e tatau ona fa'aopoopo mulimuli ile faiga fa'afoliga e fa'atumauina ai le gaioiga.
Fa'ata'ita'iga 1(Biosynthesis o mea e maua mai le phenylalanine mai mea e maua mai le phenylpyruvate)(1):
Taofi le 2 tausaga i lalo -20℃.
Aua ne'i fa'afeso'ota'i i tulaga ogaoga e pei o le: maualuga le vevela, maualuga/maualalo le pH ma le maualuga o le fa'aogaina o so'o.
1. Francesca Paradisi, Stuart Collins, et al.Journal of Biotechnology, 2007, 128, 408–411.