Nitrile reductase (NRED)
| Enzima | Fa'ailoga o le Oloa | Fa'amatalaga |
| Fa'amatalaga | ES- NRED -101~ ES- NRED -103 | o se seti o le 3 Nitrile Reductase, 50 mg i le seti 3 mea * 50mg / mea, po'o se isi lava aofa'i |
E mafai ona fa'aaogaina le faiga o tali atu lea mo se fa'asinomaga:
1-10 mg/ml Substrate
10 mg/ml Enzyme
10% (v/v) Fa'aola fa'aolaola (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Kulukose
2 mg/ml Glucose dehydrogenase (GDH)
Fa'apuna ia tali i se masini lūlū e tumau le vevela (mo se fa'ata'ita'iga, 30oC, 150rpm) mo le 24~48 itula. Fa'asolo i totonu o le centrifuge paluga ta'itasi e fa'aputu ai porotini, ona mafai lea ona fa'aogaina le supernatant mo le au'ili'iliga o le TLC、HPLC po'o le GC.
Fa'amatalaga (1): O le vaila'au fa'aola (e pei o le DMSO, methanol, acetonitrile, IPA, ma isi) e le mana'omia, e fa'aaogaina mo mea e le mafai ona solo.
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