Esterase ma Lipase (PLE ma CALB)
E 26 ituaiga oloa o le PLE enzyme (Numera o le ES-PLE-101～ES-PLE-126) na atiae e SyncoZymes. E mafai ona faʻaaogaina le ES-PLE mo le hydrolysis o aliphatic ma ester compounds, poʻo le regioselective ma le stereoselective resolution e faʻaputu ai chiral acids ma a latou mea e maua mai ai.
Ituaiga o tali fa'a-catalytic:
★ Maualuga le tulaga fa'apitoa o le substrate.
★ Filifiliga malosi o le chiral.
★ Liuga maualuga.
★ E itiiti ifo oloa e maua mai ai.
★ Tulaga o le tali atu e le ogaoga.
★ Fa'alelei i le siosiomaga.
➢ E masani lava, e tatau ona aofia i le faiga o le tali atu le substrate, le buffer solution ma le ES-PLE. O le esterification o nisi ES-PLE e faia i le organic phase.
➢ E tatau ona suʻesuʻeina taʻitasi ituaiga uma o ES-PLE e fetaui ma tulaga eseese o le tali atu sili ona lelei.
Faʻataʻitaʻiga 1 (Faʻasologa o le Pregabalin Intermediate)(1):
Fa'ata'ita'iga 2(2):
Fa'ata'ita'iga 3(3):
Fa'ata'ita'iga 4(4):
Tausi mo le 2 tausaga i lalo ifo o le -20℃.
Aua lava ne'i pa'i atu i ni tulaga ogaoga e pei o le: vevela maualuga, pH maualuga/maualalo.
1. Xu FX, Chen SY, Xu G, ma isi. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917
