Esterase&Lipase (PLE&CALB)
E 26 ituaiga o PLE enzyme oloa (Numera pei ES-PLE-101~ES-PLE-126) na atiaʻe e SyncoZymes.E mafai ona fa'aogaina le ES-PLE mo le fa'asusuina o mea fa'apipi'i aliphatic ma ester, po'o le fa'atonuga ma le fa'ata'ita'iga fa'ata'ita'iga e fa'apipi'i ai le kiral acids ma a latou mea e maua mai ai.
Ituaiga tali fa'amamafa:
★ maualuga substrate specificity.
★ Malosi le filifilia o chiral.
★ Liliu maualuga.
★ Faʻaitiitia mea faʻatau.
★ Tulaga tali malie.
★ Siosiomaga.
➢ E masani lava, o le faiga e tali atu ai e tatau ona aofia ai le mea'ai, vaifofo pa'u ma le ES-PLE.O le esterification o nisi ES-PLE o loʻo faʻatinoina i le vaega faʻasolosolo.
➢ O ituaiga uma o ES-PLE e fetaui ma tulaga eseese o tali sili ona lelei e tatau ona su'esu'eina ta'ito'atasi.
Faataitaiga 1(Biosynthesis of Pregabalin Intermediate)(1):
Faataitaiga 2(2):
Faataitaiga 3(3):
Faataitaiga 4(4):
Taofi le 2 tausaga i lalo -20℃.
Aua ne'i fa'afeso'ota'i i tulaga ogaoga e pei o le: vevela maualuga, maualuga/maualalo pH.
1. Xu FX, Chen SY, Xu G, e tal.Appl.Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron: Asymmetry 2000, 11, 917