Ene reductase (ERED)
E fa'atino e ES-EREDs ituaiga eseese o substrate ona o le lautele o le spectrum o substrates. I se tulaga lautele, o le C=C o α, β-unsaturated compounds ma electron-absorbing groups (e aofia ai le ketone aldehyde, nitro groups, carboxylic acids, esters, anhydride, lactones, imines, ma isi) e faigofie ona fa'aitiitia e ES-EREDs, ae o unactivated double bonds e leai.
E 46 ituaiga o oloa o le enzyme ERED (Numera o le ES-ERED-101~ES-ERED-146) na atiaeina e SyncoZymes.
Faiga fa'aolaola:
| Enzima | Fa'ailoga o le Oloa | Fa'amatalaga |
| Pauta Enzyme | ES-ERED-101~ ES-ERED-146 | o se seti o Ene Reductases e 46, 50 mg i le seti 46 mea * 50mg / mea, po'o se isi lava aofa'i |
| Meafaigaluega mo le Su'esu'ega (SynKit) | ES-ERED-4600 | o se seti o Ene Reductases e 46, 50 mg i le seti 46 mea * 50mg / mea, po'o se isi lava aofa'i |
★ Maualuga le tulaga fa'apitoa o le substrate.
★ Filifiliga malosi o le chiral.
★ Liuga maualuga.
★ E itiiti ifo oloa e maua mai ai.
★ Tulaga o le tali atu e le ogaoga.
★ Fa'alelei i le siosiomaga.
★ Saogalemu maualuga.
➢ E masani lava, e tatau ona aofia i le faiga o le tali atu le substrate, le buffer solution (pH sili ona lelei o le tali atu), coenzymes (NAD(H) po'o le NADP(H) ), le faiga o le toe fa'afouina o le coenzyme (e pei o le glucose ma le glucose dehydrogenase) ma le ES-ERED.
➢ E mafai ona fa'ata'ita'iina uma ES-ERED i le faiga o tali atu o lo'o i luga pe fa'aaoga le ERED Screening Kit (SynKit ERED).
➢ E tatau ona suʻesuʻeina taʻitasi ituaiga uma o ES-ERED e fetaui ma tulaga eseese o le tali atu sili ona lelei.
➢ O le substrate po'o le oloa e maualuga le malosi e ono taofia ai le gaioiga a le ES-ERED. Peita'i, e mafai ona fa'aitiitia le fa'alavelave e ala i le fa'aopoopoina o le substrate i se vaega tele.
Fa'ata'ita'iga 1(α,β-unsaturated aldehydes po'o ketones)(1):
Faʻataʻitaʻiga 2(α,β-unsaturated carboxylic acids ma a latou mea e maua mai ai)(2):
Tausi mo le 2 tausaga i lalo ifo o le -20℃.
Aua lava ne'i pa'i atu i ni tulaga ogaoga e pei o le: vevela maualuga, pH maualuga/maualalo ma le vaila'au fa'aola e maualuga le malosi.
1. Lucídio C, Fardelone J, Augusto R, e tal. J.Mol.Catal.B: Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, ma isi. .Org.Lett, 2007, 9(26): 5409-5411.
