Ene reductase (ERED)
O le ES-ERED e fa'apipi'iina ituaiga mea'ai eseese ona o le lautele o alaleo o mea'ai.I se tulaga lautele, o le C = C o le α, β -unsaturated faʻatasi ma vaega eletise-absorbing (e aofia ai le ketone aldehyde, vaega nitro, carboxylic acids, esters, anhydride, lactones, imines, ma isi) e faigofie ona faʻaititia e ES-EREDs, ae o fusi fa'alua e le'i fa'agaoioia e leai.
E 46 ituaiga o oloa enzyme ERED (Numera e pei o ES-ERED-101~ES-ERED-146) atiaʻe e SyncoZymes.
Catalytic masini:
| Enzymes | Code o oloa | Fa'amatalaga |
| Enzyme Powder | ES-ERED-101~ ES-ERED-146 | se seti o 46 Ene Reductases, 50 mg taʻitasi 46 mea * 50mg / mea, poʻo isi aofaʻi |
| Pusa su'esu'e (SynKit) | ES-ERED-4600 | se seti o 46 Ene Reductases, 50 mg taʻitasi 46 mea * 50mg / mea, poʻo isi aofaʻi |
★ maualuga substrate specificity.
★ Malosi le filifilia o chiral.
★ Liliu maualuga.
★ Faʻaitiitia mea faʻatau.
★ Tulaga tali malie.
★ Siosiomaga.
★ saogalemu maualuga.
➢ E masani lava, o le faiga e tali atu ai e tatau ona aofia ai le mea'ai, vaifofo pa'u (le tali sili ona lelei pH), coenzymes(NAD(H) po'o le NADP(H) ), coenzyme regeneration system(eg kulukose ma glucose dehydrogenase) ma ES-ERED.
➢ E mafai ona fa'ata'ita'i uma ES-ERED i le faiga e tali atu ai i luga po'o le ERED Screening Kit (SynKit ERED).
➢ O ituaiga uma o ES-ERED e fetaui ma tulaga lelei o tali e tatau ona su'esu'eina ta'ito'atasi.
➢ Fa'asa'o maualuga le mea'ai po'o se oloa e mafai ona fa'alavelaveina le gaioiga a le ES-ERED.Ae ui i lea, e mafai ona faʻamalolo le faʻalavelave e ala i le faʻaopoopoina o se vaega o substrate.
Fa'ata'ita'iga 1(α,β-aldehydes e le'o tumu po'o ketone)(1):
Fa'ata'ita'iga 2(α,β-ga'o ga'o ga'o ma mea e maua mai ai)(2):
Taofi le 2 tausaga i lalo -20℃.
Aua ne'i fa'afeso'ota'i i tulaga ogaoga e pei o le: maualuga le vevela, maualuga/maualalo le pH ma le maualuga o le fa'aogaina o so'o.
1. Lucídio C, Fardelone J, Augusto R, e tal.J.Mol.Catal.B: Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, e tal..Org.Lett, 2007, 9(26): 5409-5411.
