β-Nicotinamide adenine dinucleotide phosphate hydrate (NADP)
O le SyncoZymes (Shanghai) Co., Ltd. o le kamupani gaosi oloa ma le fa'atau oloa ta'imua i le β-Nicotinamide adenine dinucleotide phosphate hydrate (CAS:53-59-8) ma le tulaga maualuga. E mafai ona matou tu'uina atu le COA, tilivaina i le lalolagi atoa, ma le tele o aofa'iga laiti ma tele. Afai e te fiafia i lenei oloa, fa'amolemole lafo mai fa'amatalaga auiliili e aofia ai le numera CAS, igoa o le oloa, ma le aofa'i. Fa'amolemole fa'afeso'ota'i:lchen@syncozymes.com
| Igoa o le Vailaau | β-Nicotinamide adenine dinucleotide phosphate hydrate |
| Upu tutusa | β-Nicotinamide adenine dinucleotide phosphate hydrate |
| Numera CAS | 53-59-8 |
| Mamafa Molekiula | 743.41 |
| Fa'asologa Molekiula | C21H28N7O17P3 |
| Numera o le EINECS. | 200-178-1 |
| vevela o le teuina | Teu i se nofoaga pogisa, siosiomaga e lē gaoioi, teu i totonu o le pusaaisa, i lalo ifo o le -20°C |
| mafai ona solo | H2O: 50 mg/mL, manino, samasama teisi |
| pka | pKa1 3.9; pKa2 6.1 (i le 25℃) |
| λmax | 260nm (tusitusiga) |
| Merck | 14,6348 |
| Faiga Resitala o Vailaau a le EPA | Adenosine 5'-(trihydrogen diphosphate), 2'-(dihydrogen phosphate), P'.fwdarw.5'-ester fa'atasi ai ma le 3-(aminocarbonyl)-1-.beta.-D-ribofuranosylpyridinium, masima i totonu (53-59-8) |
| Mea Su'ega | Fa'amatalaga auiliili |
| Foliga vaaia | Pa'u pa'epa'e i le samasama |
| Fa'asoloatoa | 200mg/ml i le vai |
| Tau o le pH (100mg/ml) | 5.0～7.0 |
| Su'esu'ega o le UV Spectral εi le 260 nm ma le pH 7.5 | (18±1.0)×10³L/mol/cm |
| Anotusi (e ala i le auiliiliga enzymatic ma le G6(PDH ile pH 7.5, fa'aaoga le spectrophotometer, abs.340nm, i luga ole fa'avae e leai se vai) | ≥90.0% |
| Mama (e ala i le HPLC, %eria) | ≥95.0% |
| Aotelega o le vai (e KF) | ≤5% |
Afifi:Fagu, taga pepa alumini, 25kg/Papa La'au, po'o le tusa ai ma mana'oga o le tagata fa'atau.
Tulaga o le Teuina:Taofi mau i le pogisa, ae afai e te teuina mo se taimi umi, ia teu i le 2~8℃.
O le NADP o se coenzyme, o se mea lea e fusifusia ai le nicotinamide adenine dinucleotide ma le phosphate molecule e ala i se ester bond, ma e taatele lava lona iai.i le lalolagi o meaola. O ona meatotino fa'akemikolo, absorption spectrum, redox form, ma isi mea e tutusa ma le NAD (Coenzyme I). E mafai ona fa'aaogaina lautele le NADP i tali atu.e fa'atinoina e le tele o alcohol dehydrogenases, ketoreductases ma isi oxidoreductases. E pei o le: e mafai ona fa'aitiitia i le NADPH e le tele o dehydrogenases e pei oe pei o le 6-phosphoglucose dehydrogenase (EC.1.1.1.49), 6-phosphogluconate dehydrogenase (EC.1.1.1.44). Ae ui i lea, e le o lona uiga e mafai ona tali atu ma le tele oo dehydrogenases e faʻaaogaina le NAD, e le mafai foʻi ona faʻaʻoksizenina saʻo e le filifili manava. E le pei o le NAD, e tele lava ina iai i se tulaga faʻaitiitia i totonu o sela o meaola e leai ni aerobic.
E tusa ai ma le faʻaaogaina o oloa, e mafai ona vaevaeina i ni vasega nei: vasega biotransformation, vasega reagent diagnostic, vasega meaai maloloina.
Vasega o le biotransformation: E mafai ona faʻaaogaina mo le faʻapipiʻiina o vailaʻau faʻapitoa ma API, e masani lava i enzymes catalytic, e pei o le ketoreductase (KRED),P450 monooxygenase (CYP), formate dehydrogenase (FDH), glucose dehydrogenase (GDH), ma isi. Faatali.
Vasega o le reagent fa'ama'i: Faatasi ai ma le tele o enzymes fa'ama'i, e pei o le mea mata o pusa fa'ama'i.
Tulaga tau mea'ai maloloina: O le fa'aogaina o le NADP i mea'ai fa'aopoopo e tele lava ina faia i su'esu'ega ma atina'e.
